We provide precision molecular cloning services, including construct design, vector engineering, and sequence validation. Expertise spans traditional restriction/ligation strategies as well as Gibson and Golden Gate assembly methods. Optimized for microbial, plant and mammalian expression systems.
Custom CRISPR-Cas9 genome editing solutions, including guide RNA design, donor template synthesis, and clonal line generation. Experience includes primary cells, immortalized lines, and iPSC-derived systems. Screening and validation by NGS, qPCR, and functional assays.
Analysis pipelines for high-dimensional datasets including RNA-seq, WGS, and flow cytometry. Machine learning model development and feature engineering for clinical prediction tasks. Fluency in Python, R, and scientific computing environments ensures reproducibility and scalability.
Comprehensive support for Western blotting, ELISA, and related immunodetection assays. Services include antibody validation, signal optimization, quantitative analysis, and multiplex assay development. Experienced with both exploratory and high-throughput experimental contexts across cell and tissue models.
Assistance in SOP generation, troubleshooting experimental workflows, and adapting cutting-edge methods to fit lab-specific constraints. Ideal for groups launching new platforms or refining low-yield processes. Experience spans academic, clinical, and industrial settings.
Support for manuscript preparation, figure generation, grant editing, and literature reviews. Services emphasize clarity, technical rigor, and alignment with journal or agency standards. Extensive experience includes NIH grant mechanisms and high-impact peer-reviewed publications.
Leafless
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